GB 7163-1987 核反应堆保护系统的可靠性分析要求
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基本信息
| 标准名称： | 核反应堆保护系统的可靠性分析要求 |
| 中标分类： |
能源、核技术 >>
核反应堆 >>
核反应堆与 核电厂核岛设备 |
| 替代情况： | 被GB/T 7163-1999代替 |
| 发布日期： | 1900-01-01 |
| 实施日期： | 1987-10-01 |
| 首发日期： | 1900-01-01 |
| 作废日期： | 1900-01-01 |
| 出版日期： | 1900-01-01 |
| 页数： | 3页 |
适用范围
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前言
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目录
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引用标准
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所属分类： 能源 核技术 核反应堆 核反应堆与 核电厂核岛设备
【英文标准名称】:StandardGuideforDeterminingDNASingle-StrandDamageinEukaryoticCellsUsingtheCometAssay
【原文标准名称】:用彗星分析法测定真核细胞中DNA单线损伤的标准指南
【标准号】:ASTME2186-2002a
【标准状态】:现行
【国别】:
【发布日期】:2002
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.02
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:损伤;电池;核酸;遗传学
【英文主题词】:biomarker;cellular;Cometassay;DNAdamage;DNAstrandbreaks;stresseffects;toxic
【摘要】:1.1Thisguidecoverstherecommendedcriteriaforperformingasingle-cellgelelectrophoresisassay(SCG)orCometassayforthemeasurementofDNAsingle-strandbreaksineukaryoticcells.TheCometassayisaverysensitivemethodfordetectingstrandbreaksintheDNAofindividualcells.ThemajorityofstudiesutilizingtheCometassayhavefocusedonmedicalapplicationsandhavethereforeexaminedDNAdamageinmammaliancellsinvitroandinvivo(1-4).ThereisincreasinginterestinapplyingthisassaytoDNAdamageinfreshwaterandmarineorganismstoexploretheenvironmentalimplicationsofDNAdamage.1.1.1TheCometassayhasbeenusedtoscreenthegenotoxicityofavarietyofcompoundsoncellsinvitroandinvivo(5-7),aswellastoevaluatethedose-dependentanti-oxidant(protective)propertiesofvariouscompounds(3,8-11).Usingthismethod,significantlyelevatedlevelsofDNAdamagehavebeenreportedincellscollectedfromorganismsatpollutedsitescomparedtoreferencesites(12-15).StudieshavealsofoundthatincreasesincellularDNAdamagecorrespondwithhigherordereffectssuchasdecreasedgrowth,survival,anddevelopment,andcorrelatewithsignificantincreasesincontaminantbodyburdens(13,16).1.2ThisguidepresentsprotocolsthatfacilitatetheexpressionofDNAalkalinelabilesingle-strandbreaksandthedeterminationoftheirabundancerelativetocontrolorreferencecells.TheguideisageneralonemeanttofamiliarizelabpersonnelwiththebasicrequirementsandconsiderationsnecessarytoperformtheCometassay.Itdoesnotcontainproceduresforavailablevariantsofthisassay,whichallowthedeterminationofnon-alkalinelabilesingle-strandbreaksordouble-strandedDNAstrandbreaks(8),distinctionbetweendifferentcelltypes(13),identificationofcellsundergoingapoptosis(programmedcelldeath,(1,17)),measurementofcellularDNArepairrates(10),detectionofthepresenceofphotoactiveDNAdamagingcompounds(14),ordetectionofspecificDNAlesions(3,18).1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatoryrequirementspriortouse.1.3Thisguideisarrangedasfollows:SectionScope1ReferencedDocuments2Terminology3SummaryofGuide4SignificanceandUse5EquipmentandReagents6AssayProcedures7TreatmentofData8ReportingData9Keywords10AnnexA1References
